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<title>Técnica de
coloração de papanicolau</title>
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<h2 class="art-PostHeader">Técnica
de coloração de
papanicolau
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<ul style="text-align: justify;">
<li>Fixar os
esfregaços em álcool absoluto.</li>
</ul>
<ol>
<table border="1" cellpadding="4" cellspacing="0" width="100%">
<colgroup><col width="128*"><col width="128*"></colgroup><tbody>
<tr valign="top">
<td style="text-align: center;" width="50%"><strong><em>Corantes
/ reagentes</em></strong></td>
<td style="text-align: center;" width="50%"><strong><em>Tempo
/
mergulhos</em></strong></td>
</tr>
<tr valign="top">
<td width="50%">1.
Álcool 80% (+ ou -)</td>
<td width="50%">15
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">2.
Álcool 70%</td>
<td width="50%">15
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">3.
Álcool 50%</td>
<td width="50%">15
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">4.
Água destilada</td>
<td width="50%">05
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">5.
Hematoxilina de Harris</td>
<td width="50%">02 <strong>minutos</strong></td>
</tr>
<tr valign="top">
<td width="50%">6.
Água corrente</td>
<td width="50%">para
tirar o excesso de corante</td>
</tr>
<tr valign="top">
<td width="50%">7.
Solução aquosa de Hcl a 0,25%</td>
<td width="50%">02
mergulhos <strong>rápidos</strong></td>
</tr>
<tr valign="top">
<td width="50%">8.
Água corrente</td>
<td width="50%">10
minutos aproximadamente</td>
</tr>
<tr valign="top">
<td width="50%">9.
Água destilada</td>
<td width="50%">10 <strong>minutos</strong></td>
</tr>
<tr valign="top">
<td width="50%">10.
Álcool 50%</td>
<td width="50%">15
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">11.
Álcool 70%</td>
<td width="50%">15
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">12.
Álcool 80%</td>
<td width="50%">15
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">13.
Orange C-6</td>
<td width="50%">1 <strong>minuto</strong></td>
</tr>
<tr valign="top">
<td width="50%">14.
Álcool absoluto</td>
<td width="50%">15
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">15.
Álcool absoluto</td>
<td width="50%">15
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">16.
Álcool absoluto</td>
<td width="50%">15
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">17.
EA 36</td>
<td width="50%">05 <strong>minutos</strong></td>
</tr>
<tr valign="top">
<td width="50%">18.
Álcool absoluto</td>
<td width="50%">15
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">19.
Álcool absoluto</td>
<td width="50%">15
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">20.
Álcool absoluto</td>
<td width="50%">15
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">21.
Álcool absoluto</td>
<td width="50%">15
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">22.
Álcool absoluto / xilol</td>
<td width="50%">10
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">23.
1º Xilol</td>
<td width="50%">10
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">24.
2º Xilol</td>
<td width="50%">10
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">25.
3º Xilol</td>
<td width="50%">10
mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">26.
4º Xilol de montagem</td>
<td width="50%">10 <strong>minutos</strong></td>
</tr>
</tbody>
</table>
</ol>
<p style="text-align: justify;"><strong>=>
</strong>Montar a
lâmina com a lamínula e Bálsamo do
Canadá.</p>
<div style="text-align: justify;">
</div>
<p style="text-align: justify;">Cuidados:</p>
<ol style="text-align: justify;">
<li>Filtrar diariamente <span style="text-decoration: underline;"><strong>antes</strong></span>
do uso: <strong>Hematoxilina</strong>.</li>
<li>Filtar diariamente <span style="text-decoration: underline;"><strong>depois</strong></span>
do uso: <strong>Xilol de montagem</strong></li>
<li>Filtar diariamente<strong>
</strong><span style="text-decoration: underline;"><strong>depois</strong></span>
do
uso: <strong>Álcool
absoluto.</strong></li>
<li>Filtrar semanalmente os
demais corantes.</li>
</ol>
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