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Técnica de coloração de papanicolau
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<div class="art-PostHeaderIcons art-metadata-icons"><a href="http://www.cienciasdasaude.org/portal/?author=1" title="Posts de heinz" rel="author"><br>
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<ul style="text-align: justify;">
<li>Fixar os esfregaços em álcool absoluto.</li>
</ul>
<ol>
<table border="1" cellpadding="4" cellspacing="0" width="100%">
<colgroup><col width="128*"><col width="128*"></colgroup><tbody>
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<td style="text-align: center;" width="50%"><strong><em>Corantes / reagentes</em></strong></td>
<td style="text-align: center;" width="50%"><strong><em>Tempo / mergulhos</em></strong></td>
</tr>
<tr valign="top">
<td width="50%">1. Álcool 80% (+ ou -)</td>
<td width="50%">15 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">2. Álcool 70%</td>
<td width="50%">15 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">3. Álcool 50%</td>
<td width="50%">15 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">4. Água destilada</td>
<td width="50%">05 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">5. Hematoxilina de Harris</td>
<td width="50%">02 <strong>minutos</strong></td>
</tr>
<tr valign="top">
<td width="50%">6. Água corrente</td>
<td width="50%">para tirar o excesso de corante</td>
</tr>
<tr valign="top">
<td width="50%">7. Solução aquosa de Hcl a 0,25%</td>
<td width="50%">02 mergulhos <strong>rápidos</strong></td>
</tr>
<tr valign="top">
<td width="50%">8. Água corrente</td>
<td width="50%">10 minutos aproximadamente</td>
</tr>
<tr valign="top">
<td width="50%">9. Água destilada</td>
<td width="50%">10 <strong>minutos</strong></td>
</tr>
<tr valign="top">
<td width="50%">10. Álcool 50%</td>
<td width="50%">15 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">11. Álcool 70%</td>
<td width="50%">15 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">12. Álcool 80%</td>
<td width="50%">15 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">13. Orange C-6</td>
<td width="50%">1 <strong>minuto</strong></td>
</tr>
<tr valign="top">
<td width="50%">14. Álcool absoluto</td>
<td width="50%">15 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">15. Álcool absoluto</td>
<td width="50%">15 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">16. Álcool absoluto</td>
<td width="50%">15 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">17. EA 36</td>
<td width="50%">05 <strong>minutos</strong></td>
</tr>
<tr valign="top">
<td width="50%">18. Álcool absoluto</td>
<td width="50%">15 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">19. Álcool absoluto</td>
<td width="50%">15 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">20. Álcool absoluto</td>
<td width="50%">15 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">21. Álcool absoluto</td>
<td width="50%">15 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">22. Álcool absoluto / xilol</td>
<td width="50%">10 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">23. 1º Xilol</td>
<td width="50%">10 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">24. 2º Xilol</td>
<td width="50%">10 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">25. 3º Xilol</td>
<td width="50%">10 mergulhos</td>
</tr>
<tr valign="top">
<td width="50%">26. 4º Xilol de montagem</td>
<td width="50%">10 <strong>minutos</strong></td>
</tr>
</tbody>
</table>
</ol>
<p style="text-align: justify;"><strong>=> </strong>Montar a lâmina com a lamínula e Bálsamo do Canadá.</p>
<div style="text-align: justify;">
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<p style="text-align: justify;">Cuidados:</p>
<div style="text-align: justify;">
</div>
<ol style="text-align: justify;">
<li>Filtrar diariamente <span style="text-decoration: underline;"><strong>antes</strong></span> do uso: <strong>Hematoxilina</strong>.</li>
<li>Filtar diariamente <span style="text-decoration: underline;"><strong>depois</strong></span> do uso: <strong>Xilol de montagem</strong></li>
<li>Filtar diariamente<strong> </strong><span style="text-decoration: underline;"><strong>depois</strong></span> do uso: <strong>Álcool absoluto.</strong></li>
<li>Filtrar semanalmente os demais corantes.</li>
</ol>
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